PROTEOSE PEPTONE, (STD) TBL POWDER Culture Media Ingredient

Product Code: JL--2643

PHYSICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Appearance

Off white to creamish yellow colour, free flowing powder having characteristic odour but not pungent smell.

2.

Solubility (2% Soln. at 25ºC)

Completely soluble in distilled Water, Clear. Insoluble in alcohol.

3.

Clarity (2% Soln. at 121ºC.)*

Absolute clear solution. No ppt.

*- After Autoclave

 

CHEMICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

pH  (2% Soln. at 25ºC)

6.5-7.5

2.

Loss on drying (at 105ºC)

NMT-6.0%

3.

Total Nitrogen  ( DWB)

NLT- 13.0%

4.

 αAmino Nitrogen

NLT- 3.5%

5.

Total Ash

NMT-10.0%

6.

Chloride (as NaCl)

NMT-5.0%

7.

Indole Test

Positive

8.

Proteose Peptide

Positive

 

MICROBIAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Total viable count

NMT- 5,000cfu/gm

2.

Escherichia coli

Negative

3.

Salmonella spp.

Negative

4.

Staphylococcus aureus

Negative

5.

Yeast & molds

50/gm

 

GROWTH PROMOTION TEST

Cultural Characteristic observed in 2%  Proteose Peptone (STD) TBL  and 1.5% agar after incubation at 35-37°C for 18-24 hours.

S.L. No.

MICROORGANISMS

ATCC

INOCULUMS (CFU)

GROWTH

RECOVERY

1.

Staphylococcus aureus

25923

50-100

Good - Luxuriant

NLT- 70%

2.

Escherichia coli

25922

50-100

Good - Luxuriant

NLT- 70%

3.

Pseudomonas aeruginosa

27853

50-100

Good - Luxuriant

NLT- 70%

4.

Bacillus subtilis

6633

50-100

Good - Luxuriant

NLT- 70%

5.

Enterococcus faecalis

29212

50-100

Good - Luxuriant

NLT- 70%

6.

Streptococcus pyogenes

19615

50-100

Good - Luxuriant

NLT- 70%

Best Before             :  36 Months

Std. Packing          :  25kg, HDPE drum

Storage                   :  Hygroscopic by nature, store the material below 25ºC, protect from direct sunlight.


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